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Rajalakshmi, A.
- UV-visible Spectral Detection of Vaccinated Blood Samples of Sheep for Anthrax
Abstract Views :349 |
PDF Views:68
Authors
Affiliations
1 D.A.V - BHEL School, BHEL Campus, Ranipet, Vellore Dt, Tamilnadu, IN
2 P. G Dept. of Physics, Women’s Christian College, Chennai, IN
3 Registrar, Periyar University, Salem, IN
1 D.A.V - BHEL School, BHEL Campus, Ranipet, Vellore Dt, Tamilnadu, IN
2 P. G Dept. of Physics, Women’s Christian College, Chennai, IN
3 Registrar, Periyar University, Salem, IN
Source
Indian Journal of Science and Technology, Vol 2, No 5 (2009), Pagination: 49-51Abstract
UV-Visible spectroscopic technique is employed to study the spectral differences between preand post-vaccinated blood samples of sheep with anthrax spore vaccine. The internal standards among the application peaks were calculated. There was a marked difference in the absorption levels of the pre and post vaccinated blood samples. The resultant variation is attributed to the production of antibodies in the animal. Spectral study can emerge as an alternate and cost effective test for screening vaccinated animal or animal product.Keywords
Spectroscopy, Anthrax Spore Vaccine, Bacillus Anthracis, ELISA TestFull Text
References
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- A Comparative Study of Pre and Post Vaccinated Sera Samples of Bovine Using FTIR Spectral Analysis
Abstract Views :952 |
PDF Views:140
Authors
Affiliations
1 Sri Chandrasekharendra Saraswathi Viswa MahaVidyalaya, Enathur, Kanchipuram-631 561, Tamilnadu, IN
2 Registrar, Periyar University, Salem-636 011, Tamilnadu, IN
3 P.G.Department of Physics, Women’s Christian College, Chennai-600 005, Tamilnadu, IN
1 Sri Chandrasekharendra Saraswathi Viswa MahaVidyalaya, Enathur, Kanchipuram-631 561, Tamilnadu, IN
2 Registrar, Periyar University, Salem-636 011, Tamilnadu, IN
3 P.G.Department of Physics, Women’s Christian College, Chennai-600 005, Tamilnadu, IN
Source
Indian Journal of Science and Technology, Vol 3, No 4 (2010), Pagination: 382-386Abstract
This study provides scope for spectroscopic method that can be used as in-vitro test to assess the potent of vaccination instead of conventional in-vivo test. India tops in cattle wealth as well as in milk production in the world. Livestock are to be regularly vaccinated to protect them from many infectious and contagious diseases. We investigated the changes in protein and lipids levels in the pre and post vaccinated sera samples of bovine using FTIR spectral analysis. Two groups of seven animals were vaccinated with Haemorrhagic Septicaemia Vaccine (HSV) and Black Quarter Vaccine (BQV). Pre and post vaccinated sera samples of cattle were collected and tested using Fourier Transform Infra Red (FTIR) spectrometer. Spectroscopic method of blood analysis is an alternate technique to the clinical method since they require fewer samples and provide more information. The variation observed in peaks indicating the change in protein and lipids levels in the animals was due to introduce of antigens. Compared to ELISA, spectral analysis is cost effective test besides it requires small amount of sample for analysis and conforms to bioethics. In vivo challenge test can be replaced once this procedure is standardized which can satisfy the CPCSEA-"Committee for the Purpose of Control and Supervision on Experiments on Animal"- which imposes stringent regulations to use animal for experiments.Keywords
Cattle, Livestock, Vaccination, Spectroscopy, FTIR SpectrometerFull Text
References
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- Gunasekaran .S and T.S.Renuga Devi (2008) Analysis of Hyperlipidemic Patients Blood Samples – Spectral and Statistical Implications. Asian J. Clinical Cardiology, 10 (10),14-19.
- Gunasekaran S and Sankari G (2004) FTIR and UV-Visible spectral study on normal and diseased blood samples. Asian J. Chem. 16, 1779-1786.
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- Lingathurai S, Vellathurai P, Ezil Vendan S and Alwin Prem Anand A (2009) A comparative study on the microbiological and chemical composition of cow milk from different locations in Madurai, Tamil Nadu. Indian J.Sci.Technol. 2 (2), 51-54. Domain site: http://www.indjst.org.
- Renuga Devi TS, Gunasekaran S, Wesley Hudson J and Sarone Angelah Joybell I (2009) Analysis on renal failure patients blood samples: characterization and efficacy study. Indian J.Sci.Technol. 2 (2), 46-50. Domain site: http://www.indjst.org.
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- Srinivasan VA, Reddy GS, Rao KA and Ueli Kihm (2001) Serological response of bovines to combined vaccine containing foot and mouth disease virus, rabies virus, Pasteurella multocida and Clostridium chauvoei antigens. Veterinarski Arhiv. 71 (1), 37-45.
- Hepatoprotective Effect and Anti-mutagenic Activity of Zeamays (Maize) Leaves
Abstract Views :555 |
PDF Views:392
Authors
Affiliations
1 Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai- 6250021, IN
2 Department of Biochemistry and Biotechnology, Sourashtra college, Madurai-625016, IN
1 Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai- 6250021, IN
2 Department of Biochemistry and Biotechnology, Sourashtra college, Madurai-625016, IN
Source
Indian Journal of Bioinformatics and Biotechnology, Vol 2, No 1 (2013), Pagination: 35-44Abstract
The non enzymic antioxidant activities in the leaves of Zeamays were identified in four different time periods of their growth namely 10th, 15th, 20th, 25th day after their sowing. The results showed that all the non enzymic antioxidants were found to increase with the age of the leaves. The hepatoprotective effect of Zeamays leaves were analyzed by using in vivo model and inferred that CCl4 induces the liver cirrohosis and it was controlled by Zeamays leaves extract group and Vitamin-E group . The antimutagenic activity of Zeamays leaves were confirmed in Ames testKeywords
Zeamays, Non-Enzymic Antioxidants, Antimutagens, Vitamin-E, CCl4, Liver Cirrohosis, HepatotoxicFull Text
References
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- Synthesis, Characterization and Analysis of the Effect of Copper Oxide Nanoparticles in Biological Systems
Abstract Views :1137 |
PDF Views:3749
Authors
Affiliations
1 Department of plant biotechnology, School of biotechnology, Madurai kamaraj university, Madurai- 21, IN
1 Department of plant biotechnology, School of biotechnology, Madurai kamaraj university, Madurai- 21, IN
Source
Indian Journal of NanoScience, Vol 1, No 1 (2013), Pagination: 6-15Abstract
Objectives: In the present study, synthesis of copper oxide nanoparticles was prepared by chemical reduction method. This synthesized metal oxide nanoparticles were targeted to study the antibacterial activity against Escherichia coli and Staphylococcus aureus. Methods: The antibacterial activity against Escherichia coli and Staphylococcus aureus was seen by resazurin dye reduction method and disc diffusion method. Further, the effects of nanoparticles on enzyme activity (β- glucosidase) were seen in Escherichia coli. The physical interaction between metal oxides nanoparticles with protein (Ovalbumin) were studied to interrupt the conjugate forming properties. Toxicity studies of the metal oxide nanoparticles on human pathogens open the door for a new range of antibacterial agents. Results: There is a lot of interest among the researchers for developing various protocols for the fabrication of other metal nanoparticles. However, these have been a constant research regarding the use of biological systems, friendly and rapid techniques. Conclusion: The potential application of the nanomaterials-protein conjugates are limited only to novel techniques such as therapeutics in cancer, as drug delivery vehicles, smart nanocomposites, in bioremediation of industrial effluents, biodegradation of effluents and for water purification.Keywords
Copper Nanoparticles, FTIR, XRD, Resaurin Assay, Protein Nanoparticles ConjugateFull Text
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- An Improved Dynamic Data Replica Selection and Placement in Hybrid Cloud
Abstract Views :195 |
PDF Views:1
Authors
Affiliations
1 Department of Computer Science and Engineering-PG, National Engineering College, Kovilpatti, Tamilnadu, IN
1 Department of Computer Science and Engineering-PG, National Engineering College, Kovilpatti, Tamilnadu, IN
